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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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high quality camera  (Blackmagic Design Pty Ltd)
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
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multi parameter water quality sensor  (Xylem Analytics)
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DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and <t>G)</t> <t>RNA‐sequencing</t> results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).
Multi Parameter Water Quality Sensor, supplied by Xylem Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multi parameter water quality sensor - by Bioz Stars, 2026-07
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Image Search Results


DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and G) RNA‐sequencing results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).

Journal: MedComm

Article Title: DDX21 Promotes Breast Cancer Growth and Metastasis via Stimulating RNAPII Elongation During Hypoxia

doi: 10.1002/mco2.70792

Figure Lengend Snippet: DDX21 promotes HIFs‐specific target gene expression. (A–C) Volcano plots were generated to visualize global transcriptional changes between WT (20%) versus WT (1%) (A), WT (1%) versus HIFDKO (1%) (B), WT (1%) versus DDX21KD (1%) (C). (D) Multigroup volcano plots of hypoxia‐responsive genes stratified by HIF and DDX21 dependency. (E) Heatmap displaying differentially expressed genes after DDX21 knockdown and HIF knockout. (F and G) RNA‐sequencing results were validated by qPCR in MCF‐7 (F) and MDA‐MB231 (G) cell lines ( n = 3, mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).

Article Snippet: RNA extraction, RNA quality assessment, RNA library construction, sequencing, and data analysis were conducted by BGI (Beijing Genomics Institute).

Techniques: Targeted Gene Expression, Generated, Knockdown, Knock-Out, RNA Sequencing